Expression in bacteria: bacterial hosts E.coli and Bacillus
Expression in yeast: yeast expression system for the development of recombinant protein
in optimum conditions.
Expression scale: 2-10 L lab scale 25 – 100 L fermentation scale (pilot plant scale)
Protein isolation, characterization, and profiling
Protein expression analysis
Gel electrophoresis experiments- Agarose, SDS PAGE, Native page etc.
Primer efficiency test to evaluate the primer for PCR & RT-PCR