Protein Sciences

Expression in bacteria: bacterial hosts E.coli and Bacillus
 Expression in yeast: yeast expression system for the development of recombinant protein
in optimum conditions.
 Expression scale: 2-10 L lab scale 25 – 100 L fermentation scale (pilot plant scale)
 Protein isolation, characterization, and profiling
 Protein expression analysis
 Gel electrophoresis experiments- Agarose, SDS PAGE, Native page etc.
 Primer efficiency test to evaluate the primer for PCR & RT-PCR