As a foundational media, DMEM/F-12 is frequently employed in cell culture. It offers fundamental nutrients for the development of many mammalian cell lines, especially when FBS is added.
Ham’s Nutrient Mixture F-12 and Dulbecco’s Modified Eagle Medium are combined in DMEM/F-12 at a 1:1 ratio. This version of DMEM/F-12 lacks L-glutamine and has 15 mM HEPES added to it.
For applications requiring no serum, DMEM/F-12 formulations containing HEPES are typically employed. In these situations, 15 mM HEPES is added to the medium to make up for the deficient buffering capacity.
DMEM contains a buffer to maintain pH levels and usually comes with phenol red as a pH indicator. Cells cultivated in DMEM, or any other medium containing the bicarbonate buffer system, must be kept in a controlled CO2 environment of 5-10%. When phenol red is added, you can monitor pH changes from 6.2 (yellow) to 8.2 (red).
Every one of our premium cell culture media is created using the original, published recipe or with adjustments that are necessary for the culture medium to function consistently and stay stable. DMEM promotes the growth of several mammalian cell types. Along with cell lines including HeLa, HEK 293, Cos-7, and PC-12, it may be used to produce primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. The original formulation was used to cultivate embryonic mouse cells and included 1.0 g/L of glucose. It has undergone several modifications since then. Additionally, for cells with higher energy requirements, the glucose levels have been increased to 4.5 g/L.