Hanks’ Balanced Salt Solution (HBSS) containing Ca & Mg, without Phenol Red, is essential for a variety of cell culture operations, including washing cells, transferring cells or tissue samples, dilution of cells for counting, and reagent preparation.
The key functions of a balanced salt solution include maintaining pH and osmotic equilibrium, as well as providing cells with access to water and essential inorganic ions. Hanks’ Balanced Salt Solution (HBSS) can be used as a buffer to neutralize small amounts of acid or base. This serves as a stabilizer, preserving the pH of a solution.
In general, HBSS formulations containing calcium and magnesium are utilized as transport medium or to prepare reagents.