L-glutamine is a necessary amino acid and an important part of culture medium because it provides a significant amount of energy for cells in culture. When cultivated at +37°C, L-glutamine in liquid medium degrades quite quickly, and hazardous breakdown products (ammonium ions) might disrupt and potentially harm cells.
A stable dipeptide form of L-glutamine is known as stable glutamine. Stable glutamine does not spontaneously degrade into ammonia like L-glutamine does. Rather, to release L-Glutamine when required, cells break the dipeptide link. Throughout a prolonged culture, this mechanism minimises waste accumulation and ensures a constant supply of L-Glutamine.
Water suitable for cell culture is used to make this solution.