Description
RPMI (Roswell Park Memorial Institute) 1640 Advanced is a commonly used basal medium that promotes the development of mammalian cells with decreased Fetal Bovine Serum (FBS) supplementation.
Non-Essential Amino Acids (NEAA) and Sodium Pyruvate are added to this version of Advanced RPMI 1640 in place of L-Glutamine.
Serum supplementation can be decreased by 50–90% compared to standard RPMI 1640 without affecting growth rate or morphology. Sp2, Vero, Raji, Daudi, and Jurkat cells can be effectively grown in Advanced RPMI 1640 without adaptation
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